Molecular Localisation in Cells Using Fluorescence Microscopy

The course contains both theoretical and practical modules or demonstrations focusing on the different techniques used for investigating molecular localisation in cells. These include different types of microscopes, sample preparation, immunostaining and visualization, and data analysis.

The course lectures include: Fluorescence Microscopy: Introduction and description of principles; Specimen preparation; Applications; Confocal Microscopy in Biomedical Research: Different types of confocal microscopes and their uses; imaging live animals such as mice; improved resolution for examining movement of organelles and molecules in living cells; Colocalization and Interaction of Proteins: Differences between colocalization and interaction; Importance of preventing emission and excitation crosstalk; Using FRET (Fluorescence Resonance Energy Transfer) to show protein-protein interactions; Live Cell microscopy to study cellular dynamics at the molecular level; Using time series to look at translocation of proteins Laboratory topics include: Different protocols for cell fixation and permeabilization; Immunostaining of endogenous proteins and organelles; Expression and visualization of GFP-tagged proteins; RNA visualization by fluorescence in situ hybridization (FISH); Sample preparation for fluorescence microscopy; Analysis by light microscopy; Demonstration of data acquisition by confocal microscopy.

The learning forms include cathedral lectures, discussions, presentations, laboratory practicals and demonstrations. It is given at Doctoral Level (Bologna) and it corresponds to 3.0 ECTS credit points.

The course is co-organized by Cell Biology and Genetics doctoral course program and the research school of DBRM (Developmental Biology For Regenerative Medicine, www.dbrm.se). The center and the research school are funded by the Swedish Research Council(Vetenskapsrådet, www.vr.se).

• Syllabus
  
• Examination
• Schedule
• Files from lectures
• Venue
• Contact

• Doctoral course catalogue at Karolinska Institutet

The course director is Dr. Piergiorgio Percipalle and the course co-director is Dr. Emilie Louvet.

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