Nonsense-mediated Decay Practical

Check the following two splice variants for tumor necrosis factor receptor 25 by mapping them to the genomic locus. Get the protein translations and the positions of the the exon-exon junctions, and use this data to decide which one of the variants you would expect to identify in a proteomics database?

>variantA TNFR25 (nm_148965)

cgggccctgcgggcgcggggctgaaggcggaaccacgacgggcagagagc

acggagccgggaagcccctgggcgcccgtcggagggctatggagcagcgg

ccgcggggctgcgcggcggtggcggcggcgctcctcctggtgctgctggg

ggcccgggcccagggcggcactcgtagccccaggtgtgactgtgccggtg

acttccacaagaagattggtctgttttgttgcagaggctgcccagcgggg

cactacctgaaggccccttgcacggagccctgcggcaactccacctgcct

tgtgtgtccccaagacaccttcttggcctgggagaaccaccataattctg

aatgtgcccgctgccaggcctgtgatgagcaggcctcccaggtggcgctg

gagaactgttcagcagtggccgacacccgctgtggctgtaagccaggctg

gtttgtggagtgccaggtcagccaatgtgtcagcagttcacccttctact

gccaaccatgcctagactgcggggccctgcaccgccacacacggctactc

tgttcccgcagagatactgactgtgggacctgcctgcctggcttctatga

acatggcgatggctgcgtgtcctgccccacgccacccccgtcccttgcag

gagcaccctggggagctgtccagagcgctgtgccgctgtctgtggctgga

ggcagagtaggtgtgttctgggtccaggtgctcctggctggccttgtggt

ccccctcctgcttggggccaccctgacctacacataccgccactgctggc

ctcacaagcccctggttactgcagatgaagctgggatggaggctctgacc

ccaccaccggccacccatctgtcacccttggacagcgcccacacccttct

agcacctcctgacagcagtgagaagatctgcaccgtccagttggtgggta

acagctggacccctggctaccccgagacccaggaggcgctctgcccgcag

gtgacatggtcctgggaccagttgcccagcagagctcttggccccgctgc

tgcgcccacactctcgccagagtccccagccggctcgccagccatgatgc

tgcagccgggcccgcagctctacgacgtgatggacgcggtcccagcgcgg

cgctggaaggagttcgtgcgcacgctggggctgcgcgaggcagagatcga

agccgtggaggtggagatcggccgcttccgagaccagcagtacgagatgc

tcaagcgctggcgccagcagcagcccgcgggcctcggagccgtttacgcg

gccctggagcgcatggggctggacggctgcgtggaagacttgcgcagccg

cctgcagcgcggcccgtgacacggcgcccacttgccacctaggcgctctg

gtggcccttgcagaagccctaagtacggttacttatgcgtgtagacattt

tatgtcacttattaagccgctggcacggccctgcgtagcagcaccagccg

gccccacccctgctcgcccctatcgctccagccaaggcgaagaagcacga

acgaatgtcgagagggggtgaagacatttctcaacttctcggccggagtt

tggctgagatcgcggtattaaatctgtgaaagaaaacaaaacaaaacaaa

aaaaaaaaaaaaaaa

>variantB TNFR25 (nm_148968)

cgggccctgcgggcgcggggctgaaggcggaaccacgacgggcagagagc

acggagccgggaagcccctgggcgcccgtcggagggctatggagcagcgg

ccgcggggctgcgcggcggtggcggcggcgctcctcctggtgctgctggg

ggcccgggcccagggcggcactcgtagccccaggtgtgactgtgccggtg

acttccacaagaagattggtctgttttgttgcagaggctgcccagcgggg

cactacctgaaggccccttgcacggagccctgcggcaactccacctgcct

tgtgtgtccccaagacaccttcttggcctgggagaaccaccataattctg

aatgtgcccgctgccaggcctgtgatgagcaggcctcccaggtggcgctg

gagaactgttcagcagtggccgacacccgctgtggctgtaagccaggctg

gtttgtggagtgccaggtcagccaatgtgtcagcagttcacccttctact

gccaaccatgcctagactgcggggccctgcaccgccacacacggctactc

tgttcccgcagagatactgactgtgggacctgcctgcctggcttctatga

acatggcgatggctgcgtgtcctgccccacgccacccccgtcccttgcag

gagcaccctggggagctgtccagagcgctgtgccgctgtctgtggctgga

ggcagagtaggtggtgtgctgggaatgcgcgtgggagaactgggatggac

cgaggggaggcgggtgaggaggggggcaaccacccaacacccaccagctg

ctttcagtgttctgggtccaggtgctcctggctggccttgtggtccccct

cctgcttggggccaccctgacctacacataccgccactgctggcctcaca

agcccctggttactgcagatgaagctgggatggaggctctgaccccacca

ccggccacccatctgtcacccttggacagcgcccacacccttctagcacc

tcctgacagcagtgagaagatctgcaccgtccagttggtgggtaacagct

ggacccctggctaccccgagacccaggaggcgctctgcccgcaggtgaca

tggtcctgggaccagttgcccagcagagctcttggccccgctgctgcgcc

cacactctcgccagagtccccagccggctcgccagccatgatgctgcagc

cgggcccgcagctctacgacgtgatggacgcggtcccagcgcggcgctgg

aaggagttcgtgcgcacgctggggctgcgcgaggcagagatcgaagccgt

ggaggtggagatcggccgcttccgagaccagcagtacgagatgctcaagc

gctggcgccagcagcagcccgcgggcctcggagccgtttacgcggccctg

gagcgcatggggctggacggctgcgtggaagacttgcgcagccgcctgca

gcgcggcccgtgacacggcgcccacttgccacctaggcgctctggtggcc

cttgcagaagccctaagtacggttacttatgcgtgtagacattttatgtc

acttattaagccgctggcacggccctgcgtagcagcaccagccggcccca

cccctgctcgcccctatcgctccagccaaggcgaagaagcacgaacgaat

gtcgagagggggtgaagacatttctcaacttctcggccggagtttggctg

agatcgcggtattaaatctgtgaaagaaaacaaaacaaaacaaaaaaaaa

aaaaaaaaa

>genomic_TNFR25

TTGCGCTTTGCCGGACTGGATGGAGTGGAGGAGGCCCAGGCCACAGTACC

ACCCCACCTGCCCAGGCAGCCCCTCGTCACCTACTCCCCGAAGTTACCAG

CTCAGCTCGAGTCTTCAGGGCTGGGCTCCTAGGCTGCCCATCCTACTTCT

ACCCTCACTGGCCTCCAGTGGGATTCACTCCTGCCCTGCCCCCACCTTCC

CAGTCCCACAGGCCACCCCTGGCTTGGGCTGGGTTCTGTGAAGTTACGTA

TTTATTGAGCTTTTGGTTCTTTTATAAAGACTTGTCTAGACTCCACTGGG

AAGAGTCCTTTGCTTTGGGGCCCAGTGACTCGGGGCACTTGAGTTCAGGG

CGGCCTCCTTGTGTTCCTGTGCTCCTCCACTTGCCACGGATGGGCCACGG

ATGGAGCTTGCCATGGGAAGCACTGGGAAGTAATGGGGTGTGGGGTGCCA

CCAGACCAACACCCCCAGACTTCCCCACCTTCAGCCACCATCAGAGCCTC

TCCCCAGGTGCCCCCCGGGGATTCAGGGCTGAATCTGCCCAGTTCCCACA

CTCAGGCCAGCCCTCTTGGGAAGGTGGGTCCTCCATGGGGGTCCCTTCAG

GAACTTTTTTTTTTTTTTTAATACAGAGTCTCACTCTGTCACCTAGGTTG

GAGTGCAGTGGTGTGATGTCGGCTCACTGCAACCTCTGCCTCCCGGGTTC

AAACGATTCTCCTGCCCCAGCCACTCTAGTAGCTGGAACTGCAGGTGTGC

ACCACCACGCCGGGCTAGTTTTTGTATTTTAAGTAGAGACGGCATTTCAC

CATATTGGTCAGGCTGGTCTCGAACTCCTGACCCCAAGTGTTCCGCCCGC

CTCTGCCTCCCATAGTGCTAGAATTACAGGCCTGAGCTACTGCGCTTGGC

CCCTTGCGGTACTTTTGGCCCAACCTCCTCCATGGCTGGGGACGCGGAGG

CCGAGAGAGAAGTCACTTGCCCTGGCTCTACCTTGAAGTGGTTCTCAGGG

TTGGGGCGAGAGTCGGGGTGGGGACCGAGATGCAGCTCTATCCTGTGCCC

CTGGTCGCAGCAGGCAGCCCAGCGCTTCGCGTGTTCTACTTGGCCTGTCC

GCTGCCGCCTAATGAGCTCAGGTCTAGGCCGAGCAGAGGGGGCACCTGGT

CGGACTCGGTTGGGCTCGGGCGGCCCCGCCTCCCCCCGCCCGCCAGGCGG

GCCCTTCTCGACGGCGCGGGGCGGGCCCTGCGGGCGCGGGGCTGAAGGCG

GAACCACGACGGGCAGAGAGCACGGAGCCGGGAAGCCCCTGGGCGCCCGT

CGGAGGGCTATGGAGCAGCGGCCGCGGGGCTGCGCGGCGGTGGCGGCGGT

GAGTTGAGACGCAGGAGGCTAGGGGCAGCTTTGAGCCCTGTCCCAAGAGC

CTCGGTGAAGGAAGGGGCCCCGGGCGCGTCTCCACTCCAAGCCTCACTGG

GGGCTGGGTTGGCCGCCCTTTCCGATCTCGTTAAAGGCTCCTCCAGGGCG

TAGCCCTTCCACTGAGAGGAAACCCCTCCCAGGAGGGTCTGGGGGCCCCA

GGCAGAGCATTATCAGTGTCCCTCCCCCAACAGAAAGCATGGGTGGGGGT

GGGGGCGCTGCTGGATTCCTGCTCTGGTGGAGGGGAAACTTGTGAGGGGC

TGGTAAGCGCCCCCTCCGAAGCCTGGTGTGTGCGCGGGGGGAAGGAAGTT

AGTTTCCTCTCCACCCATGGGCACCCCTTCTGCCCGGGGCCTGGGAAGTG

GGCTGCTCTGTGGGCAAATGCTGGGGCCTCTGAAATGGAGGAGACGCAGC

AGGGAGAGGCCCCACGTGGGCAGCTGCGCTGAGAGTCAGCAGCACCTGTC

CCCCAGGCGCTCCTCCTGGTGCTGCTGGGGGCCCGGGCCCAGGGCGGCAC

TCGTAGCCCCAGGTGTGACTGTGCCGGTGACTTCCACAAGAAGATTGGTC

TGTTTTGTTGCAGAGGCTGCCCAGCGGGTAAGTGGCCACAGGGGTGGGAG

AGGCATGGGGCAGGCAGGGCTGGAGAGGTGGCGGGCAGGCCCGGGAGGTA

AGAGGAGGCTGGCAGGGGAGGTAGGGGTAGGCTGACAGAGAAGTAGGGAG

CTGGAGAGAAAGAGGGAGGGAGGGCAGGGTGGGAAGCAGGTCGGGGGTTG

CTGGGCAGCCCCTCTGCCTGCCTGACCCCTGCCTGGTTCCACAGGGCACT

ACCTGAAGGCCCCTTGCACGGAGCCCTGCGGCAACTCCACCTGCCTTGTG

TGTCCCCAAGACACCTTCTTGGCCTGGGAGAACCACCATAATTCTGAATG

TGCCCGCTGCCAGGCCTGTGATGAGCAGGGTGAGGGGCTTCTCAGTGCTT

GGCAGGGAGTTCCTAAGGACAGGCCTTTCTGAAGGAAGTGGCTGGCTCGG

GCCCAAACTTGGGGTGTGAGGGTCCTGCACCCACCCTTGCCAGAACCCTC

CACCCTGATCCTCCTTCAGGGTGCCCTTGCCCCTTCTCTCTTCCTGGTGA

CCTTCCCATCTCTCCATGTGCCTTGGCCTCTGGTCGGCCTTAATCTCTGA

GCTTCTCTCTTTTTTAGGGTAGCCCTGTACCTGTCTGTCTTTCGCCTATT

TCTGTCTCCATTATCTTGGGATAATGCCTCTGCCTCTCCATGGGAGCCTT

TGGCCCTGACTAACTCTCCACTCCCCATCTCCCTGCACCCCCACCAGCCT

CCCAGGTGGCGCTGGAGAACTGTTCAGCAGTGGCCGACACCCGCTGTGGC

TGTAAGCCAGGCTGGTTTGTGGAGTGCCAGGTCAGCCAATGTGTCAGCAG

TTCACCCTTCTACTGCCAACCATGCCTAGACTGCGGGGCCCTGCACCGCC

ACACACGGCTACTCTGTGAGTACCCCCACCCAGGGCTCTCTACTCCCAGA

CCCCCTTCTCCCTGCCTGACCCACTCCTGTCCCATGGTGACGCATGCCTC

TCCTGGATTGCAGGTTCCCGCAGAGATACTGACTGTGGGACCTGCCTGCC

TGGCTTCTATGAACATGGCGATGGCTGCGTGTCCTGCCCCACGTAATTCC

TAGCTGTCGTGGGATGGAGGGAAGGGCGGCTGGGAGCAGAGCAGGGGCCT

GGGGTGGGGCAGGTGCTGCTGGTTCAGGAATAGGAAGAGGGGATAGGGAG

GAGGGAGCCTTGGCCCTGTGATGGGTGGGCCCCACTTCAGGCAAACTTAG

ATGGCAAAAGAGCAATCTGGATCCGCCTTAGCCAGATACATAAGGGTATT

TGCCTTCACTTTCAGCCAGCATTCCCCCCAGCGATCCTAGCCAGATATTA

CAGATGATTTGTCACTTACACAGAGAGTCACATTGATATAGCTTTAAAAC

TTGGGCTGAAGGAGGTTGAGGCTGCAGTGAGCTATGATCGTGCCACTGCA

CTTCAGCCTGGGCAACAGAGCGAGACCTATTAAATAAATAAATAAATATT

AAATCTATTAAATATTAAATATTAAATCTATTAAATAAATAAATACAAAG

GGCTGAGAGTCAGGACTGTGCTGCTAGTTCTCTAGGGGATCTTGGGCAAG

TGCAGAGAATTCGCGTCTCTGATGTGTGGTGTCCCTTTCTCAACATGGGA

TGTTAGCAGCTAATTCACAGGCCTTTGATCAGAGGTAAGGGACTTTCTGT

AGCTATTCAAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGATGGAG

ACTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACT

ACAACCTCTGCTGCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCAA

GTAGCTGGGACTACAGGAGCCCACCACCACCCCCGGCTAATTTTTTGTAT

TTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAAGATGGTCTTGATCAC

CTGACCTCGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAG

GCATGAGCCACCGCGCCCGGCCTCCATTCAAGTCTTTATTGAATATCTGC

TATGTTCTACACACTGTTCTAGGTGCTGGGGATGCAACAGGGGACAAAAT

AGGCAAAATCCCTGTCCTTTTGGGGTTGACATTCTAGTGACTCTTCATGT

AGTCTAGAAGAAGCTCAGTGAATAGTGTCTGTGGTTGTTACCAGGGACAC

AATGACAGGAACATTCTTGGGTAGAGTGAGAGGCCTGGGGAGGGAAGGGT

CTCTAGGATGGAGCAGATGCTGGGCAGTCTTAGGGAGCCCCTCCTGGCAT

GCACCCCCTCATCCCTCAGGCCACCCCCGTCCCTTGCAGGAGCACCCTGG

GGAGCTGTCCAGAGCGCTGTGCCGCTGTCTGTGGCTGGAGGCAGAGTAGG

TGGTGTGCTGGGAATGCGAGTGGGAGAACTGGGATGGACCGAGGGGAGGC

GGGTGAGGAGGGGGGCAACCACCCAACACCCACCAGCTGCTTTCAGTGTT

CTGGGTCCAGGTGCTCCTGGCTGGCCTTGTGGTCCCCCTCCTGCTTGGGG

CCACCCTGACCTACACATACCGCCACTGCTGGCCTCACAAGCCCCTGGTT

ACTGGTAAGTACACACACCCACACACGCACCCAGAAGCCTGGGGTCAGGA

TGGGTAGCCCAGAGTCTACTCAACCCTGATACAGAAGGGGAAACTGAGGC

AGGGAGTGTGGGGTGCAGAGGAACCCTAGAGGAGCTGTACCAGCACCCAG

GTCCAGGAGGCTTGCCTGGTGGCTGACCGCAATCTCTCTGTGTCTGTCAG

CAGATGAAGCTGGGATGGAGGCTCTGACCCCACCACCGGTAAGAACCTCA

CTGTGTGATTCTGGGCTGCCTTCTGGAGCTGGAAGATCAAGCCTTACTAT

GATCCCTGGAGCTTGGCACGCGGCCAGCACCGGGTAGCCCTAGTGGACAG

AGGTGTTGGGAGCAGAGTCATCAGTGGATGAGACCAGCACAGTGCCTGCC

CTCAAGGGGTGCTCAGTCAGCTGGAGTTCAGATTCGTACACAGGAGCTAA

CAGTTCAATGGAAGGAGAGCCCCATGGTGCTGGGGGACAAGAGGAAGGAG

GCGGGGGCAGGGGACTCAAGGCAGAAGCAAGAGTTCTGCTGGGCTACAGT

GAGAGCAGGGCCAACTGTGGGAGGTGTCATTGCGGGGGTGTCTGCTGACT

GAACCAGGGACTGTCCCCTCCTGGAGAGGCACTGCGGGTAAGGGGCCTTA

CTTGGCAAGCAGGGCTGACCTGGGGCCCCTCTTGGCTTCCAGGCCACCCA

TCTGTCACCCTTGGACAGCGCCCACACCCTTCTAGCACCTCCTGACAGCA

GTGAGAAGATCTGCACCGTCCAGTTGGTGGGTAACAGCTGGACCCCTGGC

TACCCCGAGACCCAGGAGGCGCTCTGCCCGCAGGTGACATGGTCCTGGGA

CCAGTTGCCCAGCAGAGCTCTTGGTAAGGGACATCAGTGGCCTGAGGCCT

TGACCCCATTCTCCTGTCTGCGGTGGGAAGTTGTGGTTTCACAACGTGTT

CCCTTTCTGCCCCCTAACTGACGGAGTCCGCCCTATGCCCTGACCCACCG

GATCCAGCGGGCTTCAGCCCTGGGGTACCCGCACGAACGCCCCTGACTCT

GCCTCCCGACCGCGGCCCACGTACCCCAATTGGCTCTCTCTGGCCCTGCC

CCAGGCCCCGCTGCTGCGCCCACACTCTCGCCAGAGTCCCCAGCCGGCTC

GCCAGCCATGATGCTGCAGCCGGGCCCGCAGCTCTACGACGTGATGGACG

CGGTCCCAGCGCGGCGCTGGAAGGAGTTCGTGCGCACGCTGGGGCTGCGC

GAGGCAGAGATCGAAGCCGTGGAGGTGGAGATCGGCCGCTTCCGAGACCA

GCAGTACGAGATGCTCAAGCGCTGGCGCCAGCAGCAGCCCGCGGGCCTCG

GAGCCGTTTACGCGGCCCTGGAGCGCATGGGGCTGGACGGCTGCGTGGAA

GACTTGCGCAGCCGCCTGCAGCGCGGCCCGTGACACGGCGCCCACTTGCC

ACCTAGGCGCTCTGGTGGCCCTTGCAGAAGCCCTAAGTACGGTTACTTAT

GCGTGTAGACATTTTATGTCACTTATTAAGCCGCTGGCACGGCCCTGCGT

AGCAGCACCAGCCGGCCCCACCCCTGCTCGCCCCTATCGCTCCAGCCAAG

GCGAAGAAGCACGAACGAATGTCGAGAGGGGGTGAAGACATTTCTCAACT

TCTCGGCCGGAGTTTGGCTGAGATCGCGGTATTAAATCTGTGAAAGAAAA

CAAAACAAAACAAAAACGGCTTCTTGGCGTTTCTGCGGGGCTGGGGTGTT

AAGTGGACTGGACTTTTCTCGAGGGATTCGAAGGGGACGGGAATCTTGTC

ACCCCGGGATCTGGCACCCATGGTGGAGTCCAGTGTGGCCTTAGCTCCCA

AGCCTGCCCCTCCCGAGTCCACTCTGGCTCAATTACCCCGAGAAGGAGAG

AGCAAGTCGCGGCCACAGCGAGTGAGTGAACCGGAGCCCAGATGAGAGCG

CTTTAATGGGGCTGCGAGGTGGCGGAGACAGGGTCGGGATGGGGTGCAGC

AGTTGGAGACACAGGGTCAGGGCCCCTCATCCTCTATTCACTCCACCGGG

GCAGTGAAAGGGTCCCGGCAGCGAGTGGGTCTGGCTGGGGACGGGGCGCA

GACTTGAGGGAGGTGGGGCCAGAGGTGGGGTCCCGGGGGCCACAAGTGCG

GCCCGGCCAGACAGGCAGTTTTCATGTTTCTGGTAAACAAATGACTGAGA

GGAAACCCTTGGCGGGGATAAGAAATGAGGCCCGGGCTAGGCCGCGCGCA

GATCTGGGTCAGAGAGGAAGGGAAAACCCCTTCCCCGAGCTCGGGGCCTG

CCTCCACCCAGGATGCGCCAGCGACGCCGGTTCCGGGGTGCGGGGGAGGG

GGCTGCATCCTGGAGGGGGCTGAGCCCCGCAGGAAAACAAAGTCACCACC

CACGGGGCTGGGGAGAGCTCTGCACTCTTTGATAGTCAACGAACATGCAA

ATATATGTAAGTCGGCATGCAAATAAGCAGCATTTGTTG

 

-- RickardSandberg - 14 May 2008

Topic revision: r1 - 2008-05-14 - RickardSandberg

Bioinformatics for Cell Biologists (Spring -08)

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