Nonsense-mediated Decay Practical
Check the following two splice variants for tumor necrosis factor receptor 25 by mapping them to the genomic locus. Get the protein translations and the positions of the the exon-exon junctions, and use this data to decide which one of the variants you would expect to identify in a proteomics database?
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variantA TNFR25 (nm_148965)
cgggccctgcgggcgcggggctgaaggcggaaccacgacgggcagagagc
acggagccgggaagcccctgggcgcccgtcggagggctatggagcagcgg
ccgcggggctgcgcggcggtggcggcggcgctcctcctggtgctgctggg
ggcccgggcccagggcggcactcgtagccccaggtgtgactgtgccggtg
acttccacaagaagattggtctgttttgttgcagaggctgcccagcgggg
cactacctgaaggccccttgcacggagccctgcggcaactccacctgcct
tgtgtgtccccaagacaccttcttggcctgggagaaccaccataattctg
aatgtgcccgctgccaggcctgtgatgagcaggcctcccaggtggcgctg
gagaactgttcagcagtggccgacacccgctgtggctgtaagccaggctg
gtttgtggagtgccaggtcagccaatgtgtcagcagttcacccttctact
gccaaccatgcctagactgcggggccctgcaccgccacacacggctactc
tgttcccgcagagatactgactgtgggacctgcctgcctggcttctatga
acatggcgatggctgcgtgtcctgccccacgccacccccgtcccttgcag
gagcaccctggggagctgtccagagcgctgtgccgctgtctgtggctgga
ggcagagtaggtgtgttctgggtccaggtgctcctggctggccttgtggt
ccccctcctgcttggggccaccctgacctacacataccgccactgctggc
ctcacaagcccctggttactgcagatgaagctgggatggaggctctgacc
ccaccaccggccacccatctgtcacccttggacagcgcccacacccttct
agcacctcctgacagcagtgagaagatctgcaccgtccagttggtgggta
acagctggacccctggctaccccgagacccaggaggcgctctgcccgcag
gtgacatggtcctgggaccagttgcccagcagagctcttggccccgctgc
tgcgcccacactctcgccagagtccccagccggctcgccagccatgatgc
tgcagccgggcccgcagctctacgacgtgatggacgcggtcccagcgcgg
cgctggaaggagttcgtgcgcacgctggggctgcgcgaggcagagatcga
agccgtggaggtggagatcggccgcttccgagaccagcagtacgagatgc
tcaagcgctggcgccagcagcagcccgcgggcctcggagccgtttacgcg
gccctggagcgcatggggctggacggctgcgtggaagacttgcgcagccg
cctgcagcgcggcccgtgacacggcgcccacttgccacctaggcgctctg
gtggcccttgcagaagccctaagtacggttacttatgcgtgtagacattt
tatgtcacttattaagccgctggcacggccctgcgtagcagcaccagccg
gccccacccctgctcgcccctatcgctccagccaaggcgaagaagcacga
acgaatgtcgagagggggtgaagacatttctcaacttctcggccggagtt
tggctgagatcgcggtattaaatctgtgaaagaaaacaaaacaaaacaaa
aaaaaaaaaaaaaaa
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variantB TNFR25 (nm_148968)
cgggccctgcgggcgcggggctgaaggcggaaccacgacgggcagagagc
acggagccgggaagcccctgggcgcccgtcggagggctatggagcagcgg
ccgcggggctgcgcggcggtggcggcggcgctcctcctggtgctgctggg
ggcccgggcccagggcggcactcgtagccccaggtgtgactgtgccggtg
acttccacaagaagattggtctgttttgttgcagaggctgcccagcgggg
cactacctgaaggccccttgcacggagccctgcggcaactccacctgcct
tgtgtgtccccaagacaccttcttggcctgggagaaccaccataattctg
aatgtgcccgctgccaggcctgtgatgagcaggcctcccaggtggcgctg
gagaactgttcagcagtggccgacacccgctgtggctgtaagccaggctg
gtttgtggagtgccaggtcagccaatgtgtcagcagttcacccttctact
gccaaccatgcctagactgcggggccctgcaccgccacacacggctactc
tgttcccgcagagatactgactgtgggacctgcctgcctggcttctatga
acatggcgatggctgcgtgtcctgccccacgccacccccgtcccttgcag
gagcaccctggggagctgtccagagcgctgtgccgctgtctgtggctgga
ggcagagtaggtggtgtgctgggaatgcgcgtgggagaactgggatggac
cgaggggaggcgggtgaggaggggggcaaccacccaacacccaccagctg
ctttcagtgttctgggtccaggtgctcctggctggccttgtggtccccct
cctgcttggggccaccctgacctacacataccgccactgctggcctcaca
agcccctggttactgcagatgaagctgggatggaggctctgaccccacca
ccggccacccatctgtcacccttggacagcgcccacacccttctagcacc
tcctgacagcagtgagaagatctgcaccgtccagttggtgggtaacagct
ggacccctggctaccccgagacccaggaggcgctctgcccgcaggtgaca
tggtcctgggaccagttgcccagcagagctcttggccccgctgctgcgcc
cacactctcgccagagtccccagccggctcgccagccatgatgctgcagc
cgggcccgcagctctacgacgtgatggacgcggtcccagcgcggcgctgg
aaggagttcgtgcgcacgctggggctgcgcgaggcagagatcgaagccgt
ggaggtggagatcggccgcttccgagaccagcagtacgagatgctcaagc
gctggcgccagcagcagcccgcgggcctcggagccgtttacgcggccctg
gagcgcatggggctggacggctgcgtggaagacttgcgcagccgcctgca
gcgcggcccgtgacacggcgcccacttgccacctaggcgctctggtggcc
cttgcagaagccctaagtacggttacttatgcgtgtagacattttatgtc
acttattaagccgctggcacggccctgcgtagcagcaccagccggcccca
cccctgctcgcccctatcgctccagccaaggcgaagaagcacgaacgaat
gtcgagagggggtgaagacatttctcaacttctcggccggagtttggctg
agatcgcggtattaaatctgtgaaagaaaacaaaacaaaacaaaaaaaaa
aaaaaaaaa
>
genomic_TNFR25
TTGCGCTTTGCCGGACTGGATGGAGTGGAGGAGGCCCAGGCCACAGTACC
ACCCCACCTGCCCAGGCAGCCCCTCGTCACCTACTCCCCGAAGTTACCAG
CTCAGCTCGAGTCTTCAGGGCTGGGCTCCTAGGCTGCCCATCCTACTTCT
ACCCTCACTGGCCTCCAGTGGGATTCACTCCTGCCCTGCCCCCACCTTCC
CAGTCCCACAGGCCACCCCTGGCTTGGGCTGGGTTCTGTGAAGTTACGTA
TTTATTGAGCTTTTGGTTCTTTTATAAAGACTTGTCTAGACTCCACTGGG
AAGAGTCCTTTGCTTTGGGGCCCAGTGACTCGGGGCACTTGAGTTCAGGG
CGGCCTCCTTGTGTTCCTGTGCTCCTCCACTTGCCACGGATGGGCCACGG
ATGGAGCTTGCCATGGGAAGCACTGGGAAGTAATGGGGTGTGGGGTGCCA
CCAGACCAACACCCCCAGACTTCCCCACCTTCAGCCACCATCAGAGCCTC
TCCCCAGGTGCCCCCCGGGGATTCAGGGCTGAATCTGCCCAGTTCCCACA
CTCAGGCCAGCCCTCTTGGGAAGGTGGGTCCTCCATGGGGGTCCCTTCAG
GAACTTTTTTTTTTTTTTTAATACAGAGTCTCACTCTGTCACCTAGGTTG
GAGTGCAGTGGTGTGATGTCGGCTCACTGCAACCTCTGCCTCCCGGGTTC
AAACGATTCTCCTGCCCCAGCCACTCTAGTAGCTGGAACTGCAGGTGTGC
ACCACCACGCCGGGCTAGTTTTTGTATTTTAAGTAGAGACGGCATTTCAC
CATATTGGTCAGGCTGGTCTCGAACTCCTGACCCCAAGTGTTCCGCCCGC
CTCTGCCTCCCATAGTGCTAGAATTACAGGCCTGAGCTACTGCGCTTGGC
CCCTTGCGGTACTTTTGGCCCAACCTCCTCCATGGCTGGGGACGCGGAGG
CCGAGAGAGAAGTCACTTGCCCTGGCTCTACCTTGAAGTGGTTCTCAGGG
TTGGGGCGAGAGTCGGGGTGGGGACCGAGATGCAGCTCTATCCTGTGCCC
CTGGTCGCAGCAGGCAGCCCAGCGCTTCGCGTGTTCTACTTGGCCTGTCC
GCTGCCGCCTAATGAGCTCAGGTCTAGGCCGAGCAGAGGGGGCACCTGGT
CGGACTCGGTTGGGCTCGGGCGGCCCCGCCTCCCCCCGCCCGCCAGGCGG
GCCCTTCTCGACGGCGCGGGGCGGGCCCTGCGGGCGCGGGGCTGAAGGCG
GAACCACGACGGGCAGAGAGCACGGAGCCGGGAAGCCCCTGGGCGCCCGT
CGGAGGGCTATGGAGCAGCGGCCGCGGGGCTGCGCGGCGGTGGCGGCGGT
GAGTTGAGACGCAGGAGGCTAGGGGCAGCTTTGAGCCCTGTCCCAAGAGC
CTCGGTGAAGGAAGGGGCCCCGGGCGCGTCTCCACTCCAAGCCTCACTGG
GGGCTGGGTTGGCCGCCCTTTCCGATCTCGTTAAAGGCTCCTCCAGGGCG
TAGCCCTTCCACTGAGAGGAAACCCCTCCCAGGAGGGTCTGGGGGCCCCA
GGCAGAGCATTATCAGTGTCCCTCCCCCAACAGAAAGCATGGGTGGGGGT
GGGGGCGCTGCTGGATTCCTGCTCTGGTGGAGGGGAAACTTGTGAGGGGC
TGGTAAGCGCCCCCTCCGAAGCCTGGTGTGTGCGCGGGGGGAAGGAAGTT
AGTTTCCTCTCCACCCATGGGCACCCCTTCTGCCCGGGGCCTGGGAAGTG
GGCTGCTCTGTGGGCAAATGCTGGGGCCTCTGAAATGGAGGAGACGCAGC
AGGGAGAGGCCCCACGTGGGCAGCTGCGCTGAGAGTCAGCAGCACCTGTC
CCCCAGGCGCTCCTCCTGGTGCTGCTGGGGGCCCGGGCCCAGGGCGGCAC
TCGTAGCCCCAGGTGTGACTGTGCCGGTGACTTCCACAAGAAGATTGGTC
TGTTTTGTTGCAGAGGCTGCCCAGCGGGTAAGTGGCCACAGGGGTGGGAG
AGGCATGGGGCAGGCAGGGCTGGAGAGGTGGCGGGCAGGCCCGGGAGGTA
AGAGGAGGCTGGCAGGGGAGGTAGGGGTAGGCTGACAGAGAAGTAGGGAG
CTGGAGAGAAAGAGGGAGGGAGGGCAGGGTGGGAAGCAGGTCGGGGGTTG
CTGGGCAGCCCCTCTGCCTGCCTGACCCCTGCCTGGTTCCACAGGGCACT
ACCTGAAGGCCCCTTGCACGGAGCCCTGCGGCAACTCCACCTGCCTTGTG
TGTCCCCAAGACACCTTCTTGGCCTGGGAGAACCACCATAATTCTGAATG
TGCCCGCTGCCAGGCCTGTGATGAGCAGGGTGAGGGGCTTCTCAGTGCTT
GGCAGGGAGTTCCTAAGGACAGGCCTTTCTGAAGGAAGTGGCTGGCTCGG
GCCCAAACTTGGGGTGTGAGGGTCCTGCACCCACCCTTGCCAGAACCCTC
CACCCTGATCCTCCTTCAGGGTGCCCTTGCCCCTTCTCTCTTCCTGGTGA
CCTTCCCATCTCTCCATGTGCCTTGGCCTCTGGTCGGCCTTAATCTCTGA
GCTTCTCTCTTTTTTAGGGTAGCCCTGTACCTGTCTGTCTTTCGCCTATT
TCTGTCTCCATTATCTTGGGATAATGCCTCTGCCTCTCCATGGGAGCCTT
TGGCCCTGACTAACTCTCCACTCCCCATCTCCCTGCACCCCCACCAGCCT
CCCAGGTGGCGCTGGAGAACTGTTCAGCAGTGGCCGACACCCGCTGTGGC
TGTAAGCCAGGCTGGTTTGTGGAGTGCCAGGTCAGCCAATGTGTCAGCAG
TTCACCCTTCTACTGCCAACCATGCCTAGACTGCGGGGCCCTGCACCGCC
ACACACGGCTACTCTGTGAGTACCCCCACCCAGGGCTCTCTACTCCCAGA
CCCCCTTCTCCCTGCCTGACCCACTCCTGTCCCATGGTGACGCATGCCTC
TCCTGGATTGCAGGTTCCCGCAGAGATACTGACTGTGGGACCTGCCTGCC
TGGCTTCTATGAACATGGCGATGGCTGCGTGTCCTGCCCCACGTAATTCC
TAGCTGTCGTGGGATGGAGGGAAGGGCGGCTGGGAGCAGAGCAGGGGCCT
GGGGTGGGGCAGGTGCTGCTGGTTCAGGAATAGGAAGAGGGGATAGGGAG
GAGGGAGCCTTGGCCCTGTGATGGGTGGGCCCCACTTCAGGCAAACTTAG
ATGGCAAAAGAGCAATCTGGATCCGCCTTAGCCAGATACATAAGGGTATT
TGCCTTCACTTTCAGCCAGCATTCCCCCCAGCGATCCTAGCCAGATATTA
CAGATGATTTGTCACTTACACAGAGAGTCACATTGATATAGCTTTAAAAC
TTGGGCTGAAGGAGGTTGAGGCTGCAGTGAGCTATGATCGTGCCACTGCA
CTTCAGCCTGGGCAACAGAGCGAGACCTATTAAATAAATAAATAAATATT
AAATCTATTAAATATTAAATATTAAATCTATTAAATAAATAAATACAAAG
GGCTGAGAGTCAGGACTGTGCTGCTAGTTCTCTAGGGGATCTTGGGCAAG
TGCAGAGAATTCGCGTCTCTGATGTGTGGTGTCCCTTTCTCAACATGGGA
TGTTAGCAGCTAATTCACAGGCCTTTGATCAGAGGTAAGGGACTTTCTGT
AGCTATTCAAGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGATGGAG
ACTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGCACGATCTTGGCTCACT
ACAACCTCTGCTGCCTGGGTTCAAGTGATTCTCCTGCCTCAGCCTCCCAA
GTAGCTGGGACTACAGGAGCCCACCACCACCCCCGGCTAATTTTTTGTAT
TTTTAGTAGAGACGGGGTTTCACCGTGTTAGCCAAGATGGTCTTGATCAC
CTGACCTCGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAG
GCATGAGCCACCGCGCCCGGCCTCCATTCAAGTCTTTATTGAATATCTGC
TATGTTCTACACACTGTTCTAGGTGCTGGGGATGCAACAGGGGACAAAAT
AGGCAAAATCCCTGTCCTTTTGGGGTTGACATTCTAGTGACTCTTCATGT
AGTCTAGAAGAAGCTCAGTGAATAGTGTCTGTGGTTGTTACCAGGGACAC
AATGACAGGAACATTCTTGGGTAGAGTGAGAGGCCTGGGGAGGGAAGGGT
CTCTAGGATGGAGCAGATGCTGGGCAGTCTTAGGGAGCCCCTCCTGGCAT
GCACCCCCTCATCCCTCAGGCCACCCCCGTCCCTTGCAGGAGCACCCTGG
GGAGCTGTCCAGAGCGCTGTGCCGCTGTCTGTGGCTGGAGGCAGAGTAGG
TGGTGTGCTGGGAATGCGAGTGGGAGAACTGGGATGGACCGAGGGGAGGC
GGGTGAGGAGGGGGGCAACCACCCAACACCCACCAGCTGCTTTCAGTGTT
CTGGGTCCAGGTGCTCCTGGCTGGCCTTGTGGTCCCCCTCCTGCTTGGGG
CCACCCTGACCTACACATACCGCCACTGCTGGCCTCACAAGCCCCTGGTT
ACTGGTAAGTACACACACCCACACACGCACCCAGAAGCCTGGGGTCAGGA
TGGGTAGCCCAGAGTCTACTCAACCCTGATACAGAAGGGGAAACTGAGGC
AGGGAGTGTGGGGTGCAGAGGAACCCTAGAGGAGCTGTACCAGCACCCAG
GTCCAGGAGGCTTGCCTGGTGGCTGACCGCAATCTCTCTGTGTCTGTCAG
CAGATGAAGCTGGGATGGAGGCTCTGACCCCACCACCGGTAAGAACCTCA
CTGTGTGATTCTGGGCTGCCTTCTGGAGCTGGAAGATCAAGCCTTACTAT
GATCCCTGGAGCTTGGCACGCGGCCAGCACCGGGTAGCCCTAGTGGACAG
AGGTGTTGGGAGCAGAGTCATCAGTGGATGAGACCAGCACAGTGCCTGCC
CTCAAGGGGTGCTCAGTCAGCTGGAGTTCAGATTCGTACACAGGAGCTAA
CAGTTCAATGGAAGGAGAGCCCCATGGTGCTGGGGGACAAGAGGAAGGAG
GCGGGGGCAGGGGACTCAAGGCAGAAGCAAGAGTTCTGCTGGGCTACAGT
GAGAGCAGGGCCAACTGTGGGAGGTGTCATTGCGGGGGTGTCTGCTGACT
GAACCAGGGACTGTCCCCTCCTGGAGAGGCACTGCGGGTAAGGGGCCTTA
CTTGGCAAGCAGGGCTGACCTGGGGCCCCTCTTGGCTTCCAGGCCACCCA
TCTGTCACCCTTGGACAGCGCCCACACCCTTCTAGCACCTCCTGACAGCA
GTGAGAAGATCTGCACCGTCCAGTTGGTGGGTAACAGCTGGACCCCTGGC
TACCCCGAGACCCAGGAGGCGCTCTGCCCGCAGGTGACATGGTCCTGGGA
CCAGTTGCCCAGCAGAGCTCTTGGTAAGGGACATCAGTGGCCTGAGGCCT
TGACCCCATTCTCCTGTCTGCGGTGGGAAGTTGTGGTTTCACAACGTGTT
CCCTTTCTGCCCCCTAACTGACGGAGTCCGCCCTATGCCCTGACCCACCG
GATCCAGCGGGCTTCAGCCCTGGGGTACCCGCACGAACGCCCCTGACTCT
GCCTCCCGACCGCGGCCCACGTACCCCAATTGGCTCTCTCTGGCCCTGCC
CCAGGCCCCGCTGCTGCGCCCACACTCTCGCCAGAGTCCCCAGCCGGCTC
GCCAGCCATGATGCTGCAGCCGGGCCCGCAGCTCTACGACGTGATGGACG
CGGTCCCAGCGCGGCGCTGGAAGGAGTTCGTGCGCACGCTGGGGCTGCGC
GAGGCAGAGATCGAAGCCGTGGAGGTGGAGATCGGCCGCTTCCGAGACCA
GCAGTACGAGATGCTCAAGCGCTGGCGCCAGCAGCAGCCCGCGGGCCTCG
GAGCCGTTTACGCGGCCCTGGAGCGCATGGGGCTGGACGGCTGCGTGGAA
GACTTGCGCAGCCGCCTGCAGCGCGGCCCGTGACACGGCGCCCACTTGCC
ACCTAGGCGCTCTGGTGGCCCTTGCAGAAGCCCTAAGTACGGTTACTTAT
GCGTGTAGACATTTTATGTCACTTATTAAGCCGCTGGCACGGCCCTGCGT
AGCAGCACCAGCCGGCCCCACCCCTGCTCGCCCCTATCGCTCCAGCCAAG
GCGAAGAAGCACGAACGAATGTCGAGAGGGGGTGAAGACATTTCTCAACT
TCTCGGCCGGAGTTTGGCTGAGATCGCGGTATTAAATCTGTGAAAGAAAA
CAAAACAAAACAAAAACGGCTTCTTGGCGTTTCTGCGGGGCTGGGGTGTT
AAGTGGACTGGACTTTTCTCGAGGGATTCGAAGGGGACGGGAATCTTGTC
ACCCCGGGATCTGGCACCCATGGTGGAGTCCAGTGTGGCCTTAGCTCCCA
AGCCTGCCCCTCCCGAGTCCACTCTGGCTCAATTACCCCGAGAAGGAGAG
AGCAAGTCGCGGCCACAGCGAGTGAGTGAACCGGAGCCCAGATGAGAGCG
CTTTAATGGGGCTGCGAGGTGGCGGAGACAGGGTCGGGATGGGGTGCAGC
AGTTGGAGACACAGGGTCAGGGCCCCTCATCCTCTATTCACTCCACCGGG
GCAGTGAAAGGGTCCCGGCAGCGAGTGGGTCTGGCTGGGGACGGGGCGCA
GACTTGAGGGAGGTGGGGCCAGAGGTGGGGTCCCGGGGGCCACAAGTGCG
GCCCGGCCAGACAGGCAGTTTTCATGTTTCTGGTAAACAAATGACTGAGA
GGAAACCCTTGGCGGGGATAAGAAATGAGGCCCGGGCTAGGCCGCGCGCA
GATCTGGGTCAGAGAGGAAGGGAAAACCCCTTCCCCGAGCTCGGGGCCTG
CCTCCACCCAGGATGCGCCAGCGACGCCGGTTCCGGGGTGCGGGGGAGGG
GGCTGCATCCTGGAGGGGGCTGAGCCCCGCAGGAAAACAAAGTCACCACC
CACGGGGCTGGGGAGAGCTCTGCACTCTTTGATAGTCAACGAACATGCAA
ATATATGTAAGTCGGCATGCAAATAAGCAGCATTTGTTG
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RickardSandberg - 14 May 2008