Oleg Shupliakov

Group members:
Oleg Shupliakov, group leader
Åsa Winther, senior researcher
Olga Vorontsova, research engineer
Arndt Pechstein, postdoc
Anna Sundborger, postdoc
Elena Sopova, guest researcher
Richard Ordway, guest researcher
Elisabeth Kleimank, student

OLEG SHUPLIAKOV
Department of Neuroscience
Oleg.Shupliakov@ki.se

The overall goal of the research or my laboratory is to shed light on the general principles of the coordination of membrane budding events in nerve cells. This occurs during uptake (endocytosis) and degradation of receptors, synaptic vesicle recycling, during cell growth and development, and mitochondrial division. A number of viruses and bacteria utilize endocytosis to enter neurons. Malfunctions in these mechanisms lead to neurodegenerative diseases. We try to understand how the effector proteins, involved in membrane deformation and budding, become recruited to the membrane and how the action these proteins is coordinated. Our current focus is on scaffolding molecules, which coordinate effectors at the neuronal membrane.

5 SELECTED PUBLICATIONS:

von Kleist, L., Stahlschmidt, W., Bulut, H., Gromova, K.,Puchkov, D., Robertson, M.J., MacGregor, K.A., Tomlin, N., Pechstein, A., Chau, A., Chircop, M., Sakoff, J., von Kries, J., Saenger, W., Kräusslich, H-G., Shupliakov, O., Robinson, P.J., McCluskey, A., Haucke, V. (2011) Essential role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition. Cell 146:471-484.

Sundborger, A., Soderblom, C., Vorontsova, O., Evergren, E., Hinshaw, J.E., Shupliakov, O. (2011) An endophilin-dynamin complex promotes budding of clathrin-coated vesicles during synaptic vesicle recycling. J. Cell Sci. 124:133-143.

Göritz, C., Dias, D., Tomilin, N., Barbacid, M., Shupliakov, O., Frisén, J. (2011) A pericyte origin of spinal cord scar tissue. Science 333:238-242.

Pechstein A., Bacetic J., Vahedi-Faridi A., Gromova K., Sundborger A., Tomlin N., Krainer G., Vorontsova O., Schäfer J.G., Owe S.G., Cousin M.A., Saenger W., Shupliakov O.*, Haucke V.* (2010) Regulation of synaptic vesicle recycling by complex formation between intersectin 1 and the clathrin adaptor complex AP2. Proc. Natl. Acad. Sci. USA 107: 4206-4211 (*equal contribution).

Shupliakov O. (2009) The synaptic vesicle cluster: a source of endocytic proteins during neurotransmitter release. Neuroscience 158:204-210.