Sten Linnarsson

Group members:
Sten Linnarsson, group leader
Rikard Erlandsson, bioinformatician
Saiful Islam, PhD stud
Kasper Karlsson, PhD stud
Una Kjällquist, PhD stud
Peter Lönnerberg, bioinformatician
Indranil Sinha,bioinformatician
Pawel Zajac, postdoc

STEN LINNARSSON
Department of Medical Biochemistry and Biophysics (MBB)
Sten.Linnarsson@ki.se

Cell theory was developed over 150 years ago, demonstrating that all life is composed of cells, and that new cells arise exclusively from other cells. This is one of the few fundamental laws of biology. However, surprisingly there is no agreement on the number of cell types present in the mammalian body, or their relationships. One reason is the lack of tools for unbiased discovery and classification of cell types. We aim to produce a map of cell types by analyzing the transcriptomes of thousands of single cells picked at random.

We have developed methods for single-cell RNA sequencing. Our approach makes it possible to analyze the RNA in 96 single cells in a single experiment, which enables much larger experiments than have hitherto been possible. We continue to refine this method with the aim of developing a single, unified method capable of whole-transcriptome
analysis from single cells to total RNA.

5 SELECTED PUBLICATIONS:

Kivioja T, Vähärautio A, Karlsson K, Bonke M, Enge M, Linnarsson S, and Taipale, J. (2011) Counting absolute number of molecules using unique molecular identifiers. Nat. Methods, in press.

Saiful I, Kjällquist U, Moliner A, Zajac P, Fan J.B, Lönnerberg P, and Linnarsson S. (2011) Characterization of the single-cell transcriptional landscape by highly multiplex RNA-Seq. Genome Res. 21:1160-1167.

Linnarsson S. (2010) Recent advances in DNA sequencing methods - general principles of sample preparation. Exp. Cell Res. 316:1339-1343.

Pihlak A, Baurén G, Hersoug E, Metsis A, Lönnerberg P, and Linnarsson S. (2008) Rapid and inexpensive genome sequencing with short universal tiling probes. Nat. Biotechnol. 26:676-684.

Metsis A, Andersson U, Bauren G, Ernfors P, Lönnerberg P, Montelius A, Oldin M, Pihlak A, and Linnarsson S. (2004) Whole-genome expression profiling through fragment display and combinatorial gene identification. Nucleic Acids Res. 32:e127.

LINKS:

For more information, please visit our lab website at KI or here for even more info.